Biofilm questions and answers


Table of Contents

Biofilm Formation — Page 3

  • Question 1: What is the life cycle of biofilm and the mechanism of its maturation? — Page 4
  • Question 2: What surface properties favor biofilm formation? — Page 16
  • Question 3: Is the biofilm on orthopaedic implant surface permeable to neutrophils and macrophages in vivo? Are these innate immune cells (meaning any macrophages or neutrophils) capable of engulfing and killing bacteria? — Page 19
  • Question 4: Does the timescale of biofilm formation differ between bacterial species? If so, what is the timescale for common causative organisms? — Page 24
  • Question 5: Do bacteria form biofilm on the surface of cement spacer in a similar fashion to a metallic implant? — Page 27
  • Question 6: Does Mycobacterium tuberculosis form a biofilm on implants? — Page 31
  • Question 7: What is the role of the microbial synergy in polymicrobial infections? — Page 37
  • Question 8: Is the mapping of biofilm to a particular component or anatomical location an important consideration in management of implant related infections? — Page 40

Biofilm Disruption — Page 43

  • Question 1: Is there evidence that interference with bacterial communication by blocking quorum sensing molecules can minimize biofilm formation in vivo? — Page 44
  • Question 2: Can a biomaterial surface be modified to dispel bacterial adherence and biofilms? What are the potential concerns in modifying implant surfaces to combat biofilms? — Page 48
  • Question 3: What is the relevance of Minimum Inhibitory Concentration (MIC) of infecting organisms in biofilm-mediated chronic infection? — Page 58
  • Question 4: What is the Minimum Biofilm Eradication Concentration (MBEC) of anti-infective agents? — Page 63
  • Question 5: Do bacteriophages have a role in treating multidrug-resistant PJI? — Page 67