Authors: Thomas W. Bauer, Veit Krenn, Noreen Hickok, Vincent Krenn
RECOMMENDATION: The role of specific granulocyte counting methods and new immunohistologic staining techniques is to support the diagnosis of infection when diagnosis is uncertain. The recommended threshold is 5 or more polymorphonuclear leukocytes (PMNs) per field
in each of 5 high power (400x objective) magnification fields. The stains reported-to-date can only be performed on sections of formalin-fixed, paraffin embedded tissue. Therefore, they are not available for use on frozen sections obtained during an operation.
LEVEL OF EVIDENCE: Moderate
DELEGATE VOTE: Agree: 85%, Disagree: 4%, Abstain: 11% (Super Majority, Strong Consensus)