What is the role of specific granulocyte counting methods and new immunohistologic staining techniques in diagnosing periprosthetic joint infection (PJI)?

What is the role of specific granulocyte counting methods and new immunohistologic staining techniques in diagnosing periprosthetic joint infection (PJI)?

Authors: Thomas W. Bauer, Veit Krenn, Noreen Hickok, Vincent Krenn

RECOMMENDATION: The role of specific granulocyte counting methods and new immunohistologic staining techniques is to support the diagnosis of infection when diagnosis is uncertain. The recommended threshold is 5 or more polymorphonuclear leukocytes (PMNs) per field
in each of 5 high power (400x objective) magnification fields. The stains reported-to-date can only be performed on sections of formalin-fixed, paraffin embedded tissue. Therefore, they are not available for use on frozen sections obtained during an operation.

LEVEL OF EVIDENCE: Moderate

DELEGATE VOTE: Agree: 85%, Disagree: 4%, Abstain: 11% (Super Majority, Strong Consensus)

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